STUDIES ON HEMAGGLUTINATING ACTIVITY, INFECTIVITY AND IMMUNIZING ABILITY OF INFLUENZA VIRUS

III. THE ADAPTATION OF INFLUENZA VIRUS FMI TO EGGS AND MICE

Abstract

We studied the hemagglutinating activity, infectivity and immunizing ability of influenza virus FM1 (egg-adapted strain and mouse-adapted strain). The mouse-adapted strain FM1 had infectivity, immunizing ability and toxicity against chick embryo proportion to its hemagglutinating activity, but not against mice.
After we repeated the adsorption of virus in virus-suspension with chick red cells, we inoculated the 10^-1-10^-5 dilution of that non-hemagglutinating supernatant into the chorioallantoic cavity of eggs, and, after 48 hours' incubation, we recognized the virus-production by their hemagglutination. So that there may be the non-hemagglutinating but infective influenza virus.