Abstract
Application of a motion analysis system to the acute toxicity test of Daphnia was investigated.
Mobility of neonate Daphnia placed in the dilution water on a 24 wells micro culture dish was observed with a CCD camera. Observed data were analyzed using the motion analysis system “MAS”.
It turned out that neonate Daphnia magna swims constantly at the velocity about 5 mm·s-1.
It was suggested that toxicity quantification becomes possible because the loss of mobility caused by an additive corresponds to the toxicity of the additive.
The motion analysis method has an advantage as regards the short period for the test because the toxicity can be judged according to the change of mobility before death.
Furthermore, it is expected that troublesome works, the counting of the number of moving Daphnia, involved in the determination of EC50 can be omitted.
