Abstract
In order to establish standard rearing method of Red Seabream (Pargus major) for testing bioconcentration of hazardous pollutants, optimal population density, water temperature and amount of food were investigated. Necessary parameters investigated were dissolved oxygen, ammonia and pH in rearing seawater, lipid and protein contents in experimental fish, their growth ratio and their conditioning factor. The most critical parameters were dissolved oxygen and lipid content. The optimal conditions obtained using a 60l aquarium with a running water system were less than 0.59g·l-1·d-1 for population density, 20-25°C for water temperature, 4% of body weight given per day for the amount of food and 6 times a week for the frequency of feeding.