Abstract
Reaction products of chlorophenols with laccase were investigated. Chlorohydroquinone and 4-chlorocatechol of reaction products in solution obtained from reaction of 2,4-dichlorophenol with laccase were identified by GC/MS and HPLC. Similarly, dichlorodihydroxybenzene and trichlorodihydroxybenzene were estimated as a reaction product of 2,4,6-trichlorophenol and 2,3,4,6-tetrachlorophenol with laccase, respectively. The color of solution was changed to light pink after enzymatic reaction of 2,4-dichlorophenol with laccase. The color of solution disappeared after it was reduced by addition of sodium hydrosulfite. It was concluded the formation of chloro-p-benzosemiquinone caused light pink color in enzymatic reaction. This result was supported with calculated UV/VIS spectrum of chloro-p-benzosemiquinone by ZINDO program.
