Abstract
Strain TM-2 capable of growing on 4-nonylphenol (4-NP) as a sole carbon source was isolated from river water collected from Tama River.
Cultivation of strain TM-2 in a culture medium containing 4-n-nonylphenol (4-n-NP) at a concentration of 5 mg · l-1 showed that strain TM-2 could degrade 60% of 4-n-NP during the first 10 days of cultivation.
It should be noted that degradation of 4-n-NP by strain TM-2 produced an unknown compound, which was confirmed by analyzing the culture medium of strain TM-2 using a reversed phase high performance liquid chromatography equipped with an electrochemical detector (RP-HPLC-ECD). Based on the result that this electrochemically detectable compound was eluted faster than 4-n-NP on the RP - HPLC - ECD, it was suggested that a more polar phenolic compound than 4-n-NP was produced by degradation of 4-n-NP by strain TM-2.
