Abstract
The usefulness of colony formation test for the pollution evaluation of the river water was examined. Good dose-response curves were shown by the colony formation test for water samples. From the comparison with the Ames mutagenicity test, it was found that the pollutants which were combined with cytotoxicity and frameshift-type direct mutagenicity mainly presented in the river water. Furthermore, it was possible to partly clarify chemical characteristics of cytotoxic substances by being adsorbed on various solid phase adsorbents. From this fact, the colony formation test, as one of the bioassay techniques, seems to be useful for the water quality evaluation.
