Abstract
Selective cultivation of denitrifying phosphate-accumulating organisms (DNPAOs) was conducted using a sequencing batch reactor (SBR) under different electron acceptor conditions. A new biological nutrient removal process configured with anaerobic/aerobic/anoxic conditions was successfully operated in a single SBR. The supplementation of carbon substrate at the start of the aerobic condition was effective for the simultaneous removal of phosphorus and nitrate by DNPAOs.