Abstract
Water samples from Minami Asakawa River and it's draining sewages were analyzed for amino acids by high-performance liquid chromatography (HPLC).
Analytical precision and detection limit were 3.2% and 50p mol, respectively for quantitative determination of total dissolved amino acids (TDAA) and 1.5% and 5n mol, respectively for determination of amino acid composition.
The concentrations of TDAA ranged from 1.0 to 40μmol l-1 in river water and from 2.0 to 200μ mol ·l-1 in sewage water. From budget of TDAA, some parts of TDAA seemed to be removed during going down from river water.
Aspartic acid, glutamic acid, glycine and alanine were major constituents of particulate amino acids (PAA). Apparent changes in these composition were not observed during going down. However a little changes in amino acid composition of TDAA were observed during going down.
