Abstract
Effects of inorganic salts, especially of inorganic anions, were examined on the elution profiles of organic matter in sewage treated water on Sephadex G-15 with water as the eluent.
The results of this study have led to the following conclusions.
(1) The effects of inorganic salts on the gel chromatographic behavior of organic substances were principally the same as in the salt boundary technique proposed by Posner, although the salt boundaries more than one were observed in this work.
(2) The effects of anions are decisive in the elution behavior of organic substances.
(3) The principal role of inorganic salts is presumably the suppression of charge induced exclusion.
(4) Whether the sample contains inorganic salts or not, the chromatogram of organic matter with water as sole eluent may be of limited value to obtain information about the molecular-weight distribution of natural organics in aquatic environment.
(5) Even apart from the subject of the moleculer-weight distribution, the information of coexisting inorganic salts is, at least, inevitable for evaluating the quality of natural and waste on the basis of the organics.
