Abstract
In a tidal area at the estuary and the downstream of the Tamagawa river within 6km, five sampling sites were established. Moreover, two sites were established as reference, the Sekido bridge as nontidal area and the Nakayama bridge near the Lake Okutama as clean area.
The components of petrochemicals such as hexadecane, octylbenzene and 1-metylnaphthalene were added to each sample. Subsequently, they were cultivated at 20±1°C for intervals.
In result, these components were degraded to same extend at the tidal area in warm season. Hexadecane was degradaded about 80mg·l-1 for 2days and 110120mg·l-1 for 7days in summer, while octylbenzene was about 40mg·l-1 for 3days and 6070mg·l-1 for 7days in autumn. These were more degraded in warm season than cold.
And further, the biodegradation of these components were enhanced by addition of nutrients, which were constituted of NH4Cl 100mg·l-1, K2HPO4 10mg·l-1 and yeast extract 10mg·l-1.
In the existence of pentadecane, the way of biodegradation was different between octylbenzene and 1-metylnaphtalene.