Abstract
Thiocyanate degrading bacteria which were immobilized in photocrosslinked resin polymer were examined in biomass changes and specific activities of thiocyanate degradation under various culture conditions, i.e. aerated, oxygen supplied, shaken and continuous culture systems.
Biomass and specific activities of thiocyanate degradation in aerated culture system were higher than those in reciprocally shaken culture system. Oxidation rates of KSCN-S to SO42--S in the oxygen supplied and shaken culture systems were 81.9-97.9% and 80.7-84.1%, respectively, indicating oxidation of thicyanate and growth of cells in the immobilized beads have been affected by the oxygen concentration. Thiocyanate degrading bacteria formed thick mats on the surface of immobilized beads after long term cultivations under continuous and batch systems. The specific activity of thiocyanate degradation of detached immobilized beads is lower than that of non detached immobilized cells.
