Abstract
Biodegradation of tetrachloroethylene (PCE) was studied in order to explicate the source of cis-1, 2-dichloroethylene (cis-1, 2-DCE) in groundwater.
Depletion of PCE was observed by incubation with microoganisms, which lived in the soil sample from a drainage of the laundry. A bacterium which degraded PCE was isolated from this soil sample.
This bacterium (strain T) found to be an obligatory anaerobic gramnegative rod, and grew in the liquid medium containing mineral salts, yeast extract and L-cysteine monohydrochloride. The organism did not form a colony on any plate media under an anaerobic condition.
The growth of strain T and PCE degradation were observed between 15°C and 35°C, and between pH6.0 and p19.5.
PCE was transformed to TCE and then to cis-1, 2-DCE by strain T under an anaerobic condition. It was suggested that this pathway was conducted by reductive dehalogenation. Strain T could degrade more than 90% of 30mg·l-1 PCE in 60hours.
