Abstract
A bacterium which gives the high denitrifying activity at low temperature was isolated from rice field soil. Almost all the properties of the new isolate (N-1 strain) were in good agreement with those of Pseudomonas fluorescens.
For the dentirification by N-1 strain, optimum C/N ratio was about 3.5, and optimum temperature was about 25°C. Acetic acid, citric acid, and glycerin were good carbon sources for the dentirification by N-l strain.
The specific removal rate of N0-3-Nin the batch experiments at 5°C varied from 0.0140 to 0.0210 mg N0-3-N/mg Cell/hr.
