Abstract
In this study, a novel acryl fiber biomass carrier (ABC) was applied for anammox research in an up-flow column reactor. This material could retain anammox microorganism efficiently and the average weight of attached sludge in the bottom part of the reactor was calculated to be 0.14 g－TSS/cm3 ABC. High removal efficiencies of ammonium, nitrite and TN were obtained at a high volumetric loading rate of 2.0 kg－TN/m3/d. Furthermore, anammox activity did not decrease significantly with decreasing temperature, e.g., when the temperature decreased from 33.0℃ to 26.2℃, nitrite removal efficiencies decreased only about 5% (from 94% to 89%). Scanning electronic microphotographs revealed that the microorganisms were in a compacted state when attached on the support material. Based on the analysis of 16S rDNA, two close matches of anammox bacteria, KSU－1 and KOLL2a, were detected. This material showed potential for treatment of wastewater with low concentrations of nitrogen using a short hydraulic retention time.
