Role of sulfate reducing microorganisms on nitrogen removal from agricultural leachate using soil columns mixed with wood chips

Abstract

Effect of wood chips mixing soil on nitrogen removal from agriculture leacheate was examined using soil columns. Cellulose in wood chips was utilized for electron donnor for denitirification and sulfate reduction in the soil columns. Preincubation of wood chips with activated suldge was effective for increase in nitrogen removal. Microbial community of sulfate-reducing microorganisms in the soil column were examined by a nested PCR-DGGE targeting dissimilatory （bi）sulfite reductase genes. Many sulfate-reducing microorganisms are present in wood chips and rotting woods of the soil columns and incubation under denitrification condition or sulfate reducing condition resulted in a difference of microbial community. Sulfate-reducing microorganisms attached around wood may related to degradation of wood.