Abstract
This study was conducted to develop the formation and collection methods of resting cell of Anabaena macrospora, in order to clarify its physiological characteristics. A. macrospora was cultivated at water temperature of 20℃, illumination of 5,000Lx, and light-dark cycle of 12L－12D. After 4weeks cells entered the stationary phase, and resting cell began to be formed. However, resting cell began to germinate after 6weeks. By putting the medium containing both resting cells and vegetative cells in a separatory funnel, it was possible to separate precipitated resting cells from floated vegetative cells. In addition, an evaluation method of germination of A. macrospora resting cells was established by incubation of resting cells for a certain period in closed cell counting plate. We confirmed the formed resting cell had germination ability.
