Abstract
Lake Biwa has an increase of recalcitrant dissolved organic matter in the water. We examined acute toxicity of fulvic acid extracted from the lake by algal growth inhibition, crustacean immobilization, and reproduction tests to evaluate impact of recalcitrant dissolved organic matter on aquatic organisms. The results of these tests showed that present concentration of fulvic acid (FA) in Lake Biwa water did not inhibit growth of Pseudokirchneriella subcapitata, did not immobilize two Daphnia spp. and did not limit reproduction of Ceriodaphnia dubia.