Phylogenetic Analysis and 2－Methylisoborneol Production of Cyanobacterium Pseudanabaena Isolated from Drinking Water Sources in Japan

Abstract

 The phylogenetic analysis and 2－methylisoborneol production characteristics of the cyanobacterium Pseudanabaena isolated from drinking water sources in Japan were investigated. The seven strains were divided into four lineages in the 16S rRNA gene phylogenetic tree and three lineages in the 2－MIB synthase gene phylogenetic tree, and their positions in each phylogenetic tree were consistent. Culturing revealed that the strains tested grew within the temperature range of 10 to 20℃ and produced 2－methylisoborneol. Under nitrogen limination, P. limnetica WILD－11 had a higher 2－MIB content at 10℃ than at 20℃, and a significantly higher 2－MIB production ability at 10℃ than other strains. It is suggested that the ability to produce 2－MIB differs depending on the phylogeny of the 2－MIB synthase gene.