On Technical Terms in Biotechnology Related to Pulp and Paper Research (II) Methodology and Practice of Gene Manupulation Technique

Atsushi Sawatari; Shinya Kawai

doi:10.2524/jtappij.45.1363

On Technical Terms in Biotechnology Related to Pulp and Paper Research (II)

Methodology and Practice of Gene Manupulation Technique

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Keywords: Gene manupulation technique, E. coli, Restriction enzyme, Plasmid, Recombinant DNA, Transformation, Genome, Host-vector system, Gene cloning, DNA synthesizer, DNA sequencing, PAGE, HPLC, Insulin, Interferon, cDNA

JOURNAL FREE ACCESS

1991 Volume 45 Issue 12 Pages 1363-1374

DOI https://doi.org/10.2524/jtappij.45.1363

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Abstract

Methods and practice of gene manupulation technique were explained for the production of human insulin. The DNA synthesizing and DNA sequencing were outlined with a few examples. Furthermore, peculiarity in studying gene technology was also described.

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