Abstract
The purpose of this study is to clarify the effect of the traditional Japanese medicine Sairei-to (TJ-114) on edema in mice with anti-glomerular basement membrane (GBM) nephritis. Sairei-to (0.38-1.5 g/kg/day) and furosemide (50mg/kg/day) were orally administered consecutively for 4 days from day 6 after an anti-GBM serum injection. Plasma volume (PV) was determined with Evans blue dye and extracellular fluid volume (ECFV) was measured using a single-injection potassium bromide technique. In the nephritic control mice, proteinuria and hyponatreuria were induced and PV, ECFV and calculated interstitial fluid volume (ISFV) were increased. Sairei-to significantly decreased ECFV and ISFV in a dose-dependent manner, but did not change PV or urinary protein excretion. A similar result was observed in the furosemide-treated mice. These results suggest that the treatment with Sairei-to was useful against nephrotic edema.
