1993 Volume 10 Pages 117-120
The calcium-binding protein (CaBP) has been purified from rat kidney.
The purified CaBP was about 28000 molecular weight and was detected in kidney and brain. The tissue distribution and the content of CaBP were determined in normal and vitamin B6 (B6) deficient rats. In rat kidney, CaBP was present at the distal tubules and located in nuclei and cytoplasm. There was no difference in CaBP distribution between two groups. However, the content of CaBP in B6 deficient rats was significantly lower than that in normal rats. The content of serum calcium (Ca) in Ca deficient rats was significantly lower than that in Ca administrated rats. On the other hand, the content of renal CaBP in the former was significantly higher than that in the latter. Thus the content of renal CaBP was inversely proportional to the conetent of serum Ca.
We inferred that the decrease of the content of the renal CaBP was at least one factor in the formation of renal calculi observed in B6 deficient rats.
