Protective effect of dietary Vitamin C and Vitamin E against ozone induced damage of rainbow trout (Oncorhynchus mykiss).

Abstract

During the course of the study on the oxidative damage in fishes, we previously revealed that the primary target organ of ozone toxicity to fish was not gill to cause gill injury but rather red blood cells (RBC). Then decrease of blood antioxidative substances were observed. However, the effect of Vitamin C and Vitamin E against ozone-induced damage has remained unrevealed. In this study.we examined the protective effect of Vitamin C and Vitamin E against ozone induced damage of rainbow trout. The fish, weighing about 65g average, were fed experimental diets containing Vitamin C (V. C ; 100mg / 100g diets), Vitamin E (V. E; 50mg / 100g diets) and V. E-V. C mixture (V. C / E ; V. C 100mg and V. E 50mg / 100g diets) for 10 days. Level of V. C and V. E in plasma and RBC were directly reflected to diets. Fish in ozone exposure group were intermittently exposed to high concentration ozonated water (1.5ppm) for 45min. Control group turned moribund condition by ozone exposure, but V. C, V. E and V. CI E dietary group were little affected by ozon exposure. Then V. C, V. E and V. C / E suppressed RBC hemolysis, membrane lipid peroxidation and decline of Na⁺ / K⁺ ATPase activity by ozone exposure. These results indicate that dietary supplementation of V. C and V. E can be effective protection against oxidative ozone damage.