Modified High Sensitive Determination Methods of Selenium Concentration and Glutathione Peroxidase Activity in Plasma, Erythrocytes and Platelets, and These Determination in Healthy Japanese Adults.

Abstract

Modified high sensitive determination methods of selenium concentration and glutathione peroxidase (GSH-Px) activity of plasma, erythrocytes and platelets were developed. The selenium concentration and GSH-Px activity of plasma, erythrocytes and platelets in 51 healthy Japanese adults were measured, and following results were obtained. The selenium concentration (mean±SD) of plasma, erythrocytes and platelets were 117.4±15.7 μ g/L, 0.954±0.159 μ g/gHb and 4.93±1.52ng/ mg protein, respectively. The GSH-Px activity (mean±SD) of plasma, erythrocytes and platelets were 318±50U/L,18.0±5.0U/gHb and 0.142±0.035U/mg protein, respectively.