Lipid Peroxidation and Sex-dependent Differences in Iron-deficient Rats.

Abstract

The purpose of the present study was to investigate the effects of iron deficiency on lipid peroxidation and sex-dependent differences in rats. Weanling male rats (n=30) were divided into three groups of ten, and were fed on three diets : a control diet (C) ; an iron deficient diet (FeD) ; and an iron-copper deficient diet, respectively for 60 days. Lipid peroxides in the liver were determined with four methods : the three thiobarbituric acid (TBA) assay and the luminol chemiluminescence assay by using high-performance liquid chromatography (CL-HPLC), and were expressed as TBA reactive substances (TBARS) for TBA assay and phosphatidylcoline hydroperoxide (PCOOH) for CL-HPLC method. By using the TBA assay, hepatic the TBARS was decreased, but the PCOOH were higher in the FeD rats than in the control rats by the CL-HPLC method. Iron (50~ 500 μ g of Fe/ g liver as FeCl₃) was added to Iron-copper deficient liver homogenate solution. The result was that correlation between iron levels and lipid peroxide values in all methods were significant. Lipid peroxide value in Uchiyama-Mihara method was affected by the increasing of iron, especially.

Weanling male and female rats (n=40) were divided into four groups of ten, and were fed on the control diet (CM, FM) and the iron deficient diet (DM, FM), respectively for 30 days. Serum and liver TG and PL values, hepatic cytosolic XOD, GSH-Px and serum and liver PCOOH were increased in Iron deficient male rats (DM). As for female iron-deficient rats, these phenomena were not so particular without GSH-Px. It was suggested that TBARS was easy to affect by iron level in the sample solution for assay, and PCOOH accumulates as a primary peroxidation product of membrane phospholipids in liver were available for the determination of hepatic lipid peroxidation in iron deficient rat. We demonstrated that lipid peroxidation from PCOOH was occurred in iron deficiency and sex-dependent differences was detected on lipid peroxidation in iron deficient rats.