Proceeding
Effect of Ouabain on the Behavior of 28Mg in the Mice Myocardium
1997 Volume 14 Pages 29-32
Details
1997 Volume 14 Pages 29-32
Magnesium is essential for maintaining ATPase reaction which is responsible for hydrolyzing ATP to ADP. Ouabain, a specific pump inhibitor, is known to inhibits the ATPase activity. The purpose of this study was to observe the effect of ouabain on magnesium behavior in the mouse myocardium. Ouabain (0.147mg/mouse) was administered intraperitonealy to ddY male mice (6 weeks old, 25-30g) After the administration of ouabain at intervals of 5, 10, 30, 60, and 120 min., each experimental mouse received a venous injection of 28Mg via the tail. After 10 min. the mice were sacrificed. The uptake of 28Mg in the blood, heart, liver, small intestine, muscle, and bone were then measured. Autoradiography of 28Mg concentrations in the heart was also performed. 28Mg was injected 10 min. after the administration of ouabain. The mice were sacrificed 10 min. after the injection of 28Mg and uptake levels were measured. Results indicated that the administration of ouabain affected the uptake of 28Mg in the heart and small intestine. Compared to the control, the accumulation of 28Mg showed a substantial decrease within 30 min. after administration. However, after 120 min., the effect of ouabain could not be observed and the accumulation of 28Mg in the heart among the experimental group showed no significant differences. Autoradiographical analysis revealed a low accumulation of 28Mg in the ouabain treated mouse myocardium.
