Comparative studies for unit chain distributions of different glycogens and evidence of multiple branchings of oyster glycogen.

Abstract

In a series of investigation on properties of oyster glycogen, the fine structural features of glycogen was elucidated by a new analytical strategy. Thus, the multi-branched oyster glycogen molecule (A: B- chain, 0. 7: 1) involved 5 to 6 times interlinkings of B-chains. More information on the glycogen structure was investigated by peeling action by Klebsiella pullulanase. The first treatment liberated short unit-chains (dp2-5), but it was tended to produce longer unit chains (dp3-17), as the pullulanase actions proceeded. Interestingly, after the 7th treatments small proportions of longer unit-chains were still present, suggesting the presence of longer tires are in inner molecule. Based on these data, we proposed a computerized three-dimensional model of oyster glycogen.

The oyster glycogen was acted with human pancreatic amylase. The analysis by HPAEC indicated production of various maltosaccharides including double branched oligosaccharides. The aforementioned isoamylolysis and HP AEC analysis was applied to the unit chain distributions of glycogens from different biological origins. It was also successfully applied for clarification of the glycogen of human glycogen storage disease.