Participation of functional mineral-encaged zeolite treated water species in restoration of cell motility of organotin-intoxicated Euglenagracilis Z

Abstract

Series of research in our laboratory has shown that restoration of morphology and motility of TBTCl-intoxicated Euglena gracilis Z should be resulting from trace minerals in the processed aqueous solution. To examine the detoxication effect of mineral-encaged zeolite on the impaired cells, we used the bioassay system using Euglena gracilis Z as the model organism and TBTCl as the model xenobiotic. Under the polluted environment, Euglena cells changes their shape to spherical form from spindle form. By taking advantage of this unique character, we examined the different types of zeolites which encaged different minerals. TBTCl-intoxicated Euglena cells were separately washed with the water species processed by zeolite, then incubated in the processed water for up to 3 hours. The restoration of motility of the cell was estimated by observing the motile cell number using a video-microscopy system. A remarkable recovery of cell motility was observed with the incubation system using Fe, Mn and Zn encaging zeolite. However, they did not show any recovery effect when they were treated with a chelator, Chelex-100.