Preparation and characterization of antibody against glycyl-L-histidyl-L-lysine-copper (II) complex

Abstract

Antiserum against gly-L-his-L-lys (GHK)-Cu²⁺ complex has been generated using BSA-GHK-Cu²⁺ conjugate as an immunogen, and an EIA for the determination of GHK -Cu²⁺ has been developed. The standard curve allows the determination of 8×10^-7M to 1×10^-5 M of GHK-Cu²⁺. The EIA method was applied to the determination of GHK-Cu²⁺ spiked in rat plasma. Recoveries from o-phenanthroline treated rat plasma were satisfactory whereas they from untreated rat plasma were low. The antiserum can bind to not only GHK-Cu²⁺ but also GHK, though the affinity is higher for the former than for the latter.