Metal-induced histomorphological injury and the alterations in the amounts and proportion of unsaturated disaccharides of glycosaminoglycan derived from proteoglycan

Abstract

To determine the extent of the metal-induced histomorphological injury and degeneration of tissuues, the relationship between organotin-induced histomorphological injury and the alterlations in the amounts and proportion of unsaturated disaccharides of glycosaminoglycan derived from proteoglycan were analyzed by using a high performance liquid chromatography (HPLC). In this article, the alterlations in the amounts and proportion of unsaturated disaccharides of glycosaminoglycan are discussed with a focus on the organotin-exposed hippocampus and thymus. The disaccharide compositions of glycosaminoglycan from the hippocampus and thymus were significantly altered by the exposure of organotin compounds. In the hippocampus, increases in the proportion of chondroitin (CS-0S) and dermatan (DS-0S) and decreases in the proportion of chondroitin 4-sulfate (CS-4S) and dermatan 4-sulfate (DS-4S) were seen in the one-week oral exposure of tributyltin. In the thymus, an increase in the proportion of unsaturated disaccharide units (ΔDi-diSB ) derived from dermatan sulfate and a decrease in the proportion of unsaturated disaccharide units (ΔDi-0S) derived from chondroitin sulfate were seen in the one-week oral exposure of dibutyltin. An increase in the proportion of disaccharide units (ΔDi-0S) and a decrease in the proportion of disaccharide units (ΔDi-4S) were seen in the one-week oral exposure of tributyltin. These alterlations in the thymus depend on an decrease in the absolute amounts of chondroitin sulfate derived from the atrophy of the thymus which contains lot amounts of chondroitin sulfate proteoglycan.