Relationship between the reactive oxygen species and substrate oxygenation in ethanol- induced rat liver microsomes

Abstract

Cytochrome P450 is a group of enzymes that are responsible for the biotransformation of numerous endogenous and exogenous compounds. However, the precise process of dioxygen activation involved in P450-dependent substrate oxygenations has not been established. Previously, we reported that singlet oxygen (¹O₂) played an important role in microsomal P450-dependent oxygenation. In order to confirm the ¹O₂ involvement, we investigated further the relationship between the formation of reactive oxygen species (ROS) and a substrate oxygenation in the ethanol-induced rat liver microsomal P450.