Proceeding
Determination of Vanadium and Molybdenum in Biological Samples by High-Performance Liquid Chromatography
1986 Volume 3 Pages 5-12
Details
1986 Volume 3 Pages 5-12
The determinations of a trace amount of molybdenum, tungsten and vanadium by high-performance liquid chromatography are proposed.
The molybdenum and tungsten in the form of tiron ( 1, 2-dihydroxybenzene-3, 5-disulfonic acid) chelates were separated on a C18-bonded phase column with methanol-water containing tetrabutylammonium bromide (TBA-Br) as a mobile phase, and were simultaneously determined at 315 nm.
The vanadium in the form of PAR (4-(2-pyridylazo)-resorcinol) chelate was also separated in the same way as the determination of molybdenum and tungsten, and was spectrophotometrically determined at 555nm.
These techniques were applied successfully to the analysis of plant materials after wet ashing of the sample and extraction of the molybdenum and tungsten asabenzoinoxime complexes or the vanadium as the N-benzoyl-N-phenylhydroxylamine complex.
