2021 Volume 38 Pages 44-47
To clarify the role of biotin in palatal development, we investigated the effects of biotin supplementation to pregnant dams on the development of palatal processes in biotin-deficient fetal mice. Pregnant mice were administered either a biotin-deficient diet or biotin-supplemented (control) diet from day 0 of gestation (dg 0). Several mice in the biotin-deficient group were administered biotin intraperitoneally at 0900 on dg 10. After biotin administration, they were fed the biotin-supplemented diet during day 10-12, 10-13 or 10-14 of gestation, and then switched to a biotin-deficient diet until dg 15. The incidence of cleft palate was 63.6 % in the biotin-deficient group, whereas no cleft palate was noted in the other groups. This suggested that biotin is necessary for palatal development in the initial stage of palatogenesis.
