Proceeding
Studies on 45Ti-Ascorbate Biodistribution and Its Metabolism
1988 Volume 5 Pages 121-125
Details
1988 Volume 5 Pages 121-125
Titanium (Ti) is a common element widely spread in the earth’s crust, but it is not known whether Ti is an essential element or not. Pais et al. found out that titanium-ascorbate (Ti-AsA) promoted growth in some plants. We are now investigating the essentiality of Ti in animals. For this purpose, we prepared 45TiCl4 and 45Ti-AsA, and investigated their biochemical behaviors.
Guinea pigs fed experimental diets (low AsA: 28mg AsA/100g diet, high AsA: 4000mg AsA/100g diet) for two weeks, were administered with 45TiCl4 and 45Ti-AsA. The tissue uptake of 45TiCl4 was accompanied by a parallel rise of AsA level in the diet. On the other hand, the uptake of 45Ti-AsA was not enhanced by AsA level in the diet, although tissue uptake was high even in case of low AsA level in diet.
Whole-body autoradiograms were performed with 45TiCl4 or 45Ti-AsA in Wistar rat. 45Ti-AsA was well distributed rather than 45TiCl4. These data indicate that AsA promotes the transport of Ti. Metabolites of 45Ti-[14C]-AsA in the plasma of rats were analyzed by HPLC using gel filtration or ion-exchange technique. Ti-AsA mainly exists as Ti-AsA-transferrin complex in vitro.
