2008 Volume 12 Issue 1 Pages 43-50
The distribution of the adhesive capsular protein (Cp39) gene, the protein antigen, and its genetic variation among avian clinical isolates of Pasteurella multocida in Japan were determined by the gene sequencing, the PCR-restriction fragment length polymorphism (PCR-RFLP) and immunoblot analysis. The results demonstrated that the gene distributed among all the somatic serotype 3 isolates. Adhesive protein gene-based PCR-RFLP categorized the clinical isolates into three patterns by digestion with HindIII or three patterns with EcoRI, respectively. Genetic diversity of the adhesive protein gene was observed among those isolates but it did not influence their virulence to hosts. Whole-cell lysate of all clinical isolates incubated with sample buffer at 37°C were immunostained with chicken antiserum against recombinant Cp39 (rCp39) by immunoblot analysis. Therefore, the rCp39 may be an emerging candidate of effective component for fowl cholera vaccine in Japan.
