Inactivation of MS2 Phage and Cryptosporidium parvum Oocysts Using UV-A from High-Intensity Light-Emitting Diode for Water Disinfection

Atsushi HASHIMOTO; Kazuaki MAWATARI; Yohsuke KINOUCHI; Masatake AKUTAGAWA; Naotomo OTA; Kazuyuki NISHIMURA; Tsuyoshi HIRATA; Akira TAKAHASHI

doi:10.2965/jwet.2013.299

Original Papers

Inactivation of MS2 Phage and Cryptosporidium parvum Oocysts Using UV-A from High-Intensity Light-Emitting Diode for Water Disinfection

Atsushi HASHIMOTO, Kazuaki MAWATARI, Yohsuke KINOUCHI, Masatake AKUTAGAWA, Naotomo OTA, Kazuyuki NISHIMURA, Tsuyoshi HIRATA, Akira TAKAHASHI

Author information

Atsushi HASHIMOTO
Department of Life and Environmental Sciences, Prefectural University of Hiroshima, Japan
Kazuaki MAWATARI
Institute of Health Sciences, University of Tokushima, Japan
Yohsuke KINOUCHI
Institute of Technology and Sciences, University of Tokushima, Japan
Masatake AKUTAGAWA
Institute of Technology and Sciences, University of Tokushima, Japan
Naotomo OTA
Department of Civil Engineering, Anan National College of Technology, Japan
Kazuyuki NISHIMURA
Department of Life and Environmental Sciences, Prefectural University of Hiroshima, Japan
Tsuyoshi HIRATA
Department of Life and Environmental Sciences, Azabu University, Japan
Akira TAKAHASHI
Institute of Health Sciences, University of Tokushima, Japan

Keywords: Cryptosporidium, inactivation, LED, MS2 phage, UV-A

JOURNAL FREE ACCESS

2013 Volume 11 Issue 4 Pages 299-307

DOI https://doi.org/10.2965/jwet.2013.299

Details

Abstract

In this study, high-intensity, UV-A (ranging from 360 to 370 nm, peak wavelength at 365 nm) produced by a light-emitting diode was used for the inactivation of MS2 phage and Cryptosporidium parvum oocyst. In the irradiation experiment with MS2 phage, approximately 44 and 65 J/cm² of UV-A were required to obtain -2 and -3 log inactivations, respectively. The -2 and -3 log inactivations of Cryptosporidium oocysts required 338 and 508 J/cm² UV-A, respectively, which were 7.7 - 7.8 times greater than those required for MS2 phage. The possibility that high-intensity UV-A irradiation can inactivate both protozoa and viruses (phage) was demonstrated in this study.

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