Journal of Water and Environment Technology
Online ISSN : 1348-2165
ISSN-L : 1348-2165
Original Articles
Development and Characterization of a Chloroethenes-Dechlorinating Consortium Using Gluconate as a Hydrogen Donor
Yuta FujiiKazuhiro MitsukaHiroki OgataDaisuke InoueMichihiko Ike
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JOURNALS OPEN ACCESS
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2020 Volume 18 Issue 4 Pages 212-225

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Abstract

To evaluate the effectiveness of gluconate as a potential hydrogen donor for reductive dechlorination of chloroethenes, we developed a trichloroethene (TCE)-dechlorinating consortium from chloroethenes-contaminated groundwater containing Dehalococcoides using gluconate as the sole hydrogen donor and characterized its chloroethenes-dechlorinating performance and microbial community composition. After repeated subculturing, we successfully developed a microbial consortium with gluconate, which showed consistent dechlorination of TCE to vinyl chloride (VC), resulting in subsequent disappearance of VC. The resultant consortium could dechlorinate 24 μmol/L TCE to VC and eliminate VC within 20 days, which was similar to or slightly longer than the required time when other hydrogen donors such as lactate or methanol were used. These results indicate that gluconate can be an effective hydrogen donor for reductive dechlorination of chloroethenes. Real-time PCR and terminal restriction fragment length polymorphism analyses of bacterial and archaeal populations revealed the establishment of a stable microbial community in the developed consortium. In addition, 16S rRNA amplicon sequencing revealed that Trichococcus and Malikia were the predominant taxa, whereas Dehalococcoides and other dechlorinating populations were minor. Our results infer that the use of gluconate as a hydrogen donor established a distinctive microbial community compared with enriched consortia developed with other hydrogen donors.

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© 2020 Japan Society on Water Environment

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND) 4.0 License.
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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