Screening of Soil-Born Fungi from Forest Soil Using Glyphosate Herbicide as the Sole Source of Phosphorus

Abstract

Glyphosate herbicide was used as screening agent to be the sole source of phosphorus for the isolation of soil-born fungi of forest soil. Three fungal strains were able to grow consistently in the presence of glyphosate as the sole phosphorus source and were identified as Fusarium sp. strain FRP1, Scopulariopsis sp. strain FRP2 and Trichoderma sp. strain FRP3. On standard medium ten fungal strains were isolated and identified as Botrytis sp. strain FR1, Mucor sp. strain FR2, Acremonium sp. strain FR3, Trichoderma sp. strain FR4, Botrytis sp. strain FR5, Crysosporium sp. strain FR6, Scopulariopsis sp. strain FR7, Trichoderma sp. strain FR8, Botrytis sp. strain FR9 and Acremonium sp. strain FR10. Of the three screened fungal species, Scopulariopsis sp. strain FRP2 and Trichoderma sp. strain FRP3 were selected for further study because they had the highest ratio of growth diameter. The growth kinetics of Scopulariopsis sp. strain FRP2 and Trichoderma sp. strain FRP3 were also observed on broth Czapek medium containing glyphosate as the sole source of phosphorus. Synergetic interactions in vitro were examined on PDA. The synergetic interactions of Scopulariopsis sp. strain FRP2 and Trichoderma sp. strain FRP3 as mix culture showed antibiosis inhibition mode.