Abstract
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and polymerase chain reaction (PCR) using the specific primers for the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) enables us sensitive detection of basidiomycetes in decayed wood and identification of their species by BLAST analysis of DNA sequence on database. This method was applied for detection and identification of basidiomycetes in wood blocks, which had been settled in bait stations around a site of house. As the results, DNA sequences of ITS region were obtained for 103 samples from 16 bait stations. The corresponding species of basidiomycete were identified for 80 samples, which distributes 17 species in 10 genera, suggesting a high diversity of fungal flora depending on micro-environment within a site of house.
