Mokuzai Gakkaishi
Online ISSN : 1880-7577
Print ISSN : 0021-4795
ISSN-L : 0021-4795
Notes
Identification of Quercus Section Prinus Species using RAPD Markers
Seungmin LeeEri TamakiSadanobu KatohTakeshi Furuno
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2007 Volume 53 Issue 3 Pages 157-162

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Abstract
Species-specific random amplified polymorphic DNA (RAPD) markers of Quercus serrata Thunb. (konara), Quercus crispula Blume (mizunara) and Quercus alba Linn. (white oak) were investigated to identify the species of Section Prinus in the subgenus Quercus by using molecular genetic information. Polymerase Chain Reaction was performed with a set of 10 mer or 12 mer primers and total DNA as template. A total of 144 (72 sets) primers were used in the RAPD analysis. We found that six RAPD markers were species-specific : one distinguishes konara from mizunara and white oak ; four distinguish mizunara from konara and white oak ; and one distinguishes white oak from konara and mizunara. All of the six RAPD markers were highly reproducible and resulted from distinct amplicons.
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© 2007 by The Japan Wood Research Society
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