The Antioxidant Activity of the Extracts from Disposition of the Waste Sawdust Substrate from Shiitake Mushroom (Lentinula edodes) Cultivation by the Two-step Hot/hot-compressed Water Percolation

Abstract

Disposition of the waste sawdust substrate from sawdust-based cultivation of shiitake mushrooms (Lentinula edodes) is an important issue. In this study, we evaluated the physiological activity in the water extracts of waste sawdust substrate which had been extracted by the two-step hot/hot-compressed water percolation. The 180°C water extract of waste sawdust substrate contained some isoprenoid and high amounts of polyphenol. Additionally, high antioxidant activity was found in the 180°C water extract of the waste sawdust substrate in in vitro experiment (DPPH scavenging activity) and the human keratinocyte, HaCaT. This study suggested that the two-step hot/hot-compressed water percolation elutes the antioxidant components in the 180°C fraction from the waste sawdust substrate.