Factors Affecting Mycelial Growth of New Variety Pleurotus eryngii × Pleurotus nebrodensis named ON206

Abstract

To clarify the physiological characteristics of the new variety ON206, external factors affecting the nutritional growth of mycelia were investigated, particularly focusing on the initial pH, nutrient composition, and effect of light on mycelium. The optimum initial pH was 5.6, and growth was possible in a wide range of pH, from 4.0 to 8.9. As a carbon source, soluble starch showed the best growth, and the maximum mycelial volume was observed at a concentration of 15.0%. As a nitrogen source, yeast extract showed the best growth, with an optimum concentration of 2.0%. Organic nitrogen tended to be more effective for mycelial growth than inorganic nitrogen. Light affected mycelial growth of ON206. In particular, blue light tended to suppress mycelial growth and increase mycelial density. This study established the basic culture characteristics of the new variety ON206 and its nutrient requirements and photosensitivity tended to be similar to the mother strain, Pleurotus nebrodensis.