Determination of Chlornitrofen (CNP) and CNP-amino in Tap Water by Solid-phase Extraction- LC-APCI-MS/MS

Abstract

In this study, we simultaneously analyzed chlornitrofen (CNP) and CNP-amino in tap water by solid-phase extraction-LC-APCI-MS/MS, through the generation of the phenoxide ion of CNP using the negative mode of atmospheric pressure chemical ionization (APCI). As a result, the detection limit for CNP could reach as low as 1/100th of the target value (0.0001 mg/L) of a complementary item in the Japanese Waterworks Act by concentrating the sample water 100 times using solid-phase extraction. The calibration curve generated with this method showed higher accuracy and precision, and the results of the tap water spike and recovery test proved that this method met the accuracy, repeatability, and intermediate precision targets standardized by the Japanese validation guideline concerning tap water analysis, thereby supporting the validity of our method.