2021 Volume 90 Issue 5 Pages 2-12
In Japan, musty odor produced by cyanobacteria are major problems for comfortability in drinking water.Available information on morphological characteristics and gene information of musty odor producing cyanobacteria in Japan is limited. In this study, musty odor producing cyanobacteria in nationwide water sources were surveyed by optical microscope and gene analysis and collected their characteristics and gene information. Aphanizomenon spp., Dolichospermum spp. and Phormidium spp. were found as geosmin producing cyanobacteria. And, Microcoleus spp., Phormidium spp., Planktothricoides spp. and Pseudanabaena spp. were found as 2-methylisoborneol (2-MIB) producing cyanobacteria. As results of morphological characteristics and 16S rRNA gene analysis, some parts of musty odor producing cyanobacteria were unable to identified by only morphological characteristics or gene analysis. Combination between morphological characteristics and gene analysis is important to identify these cyanobacteria. In gene detection of geosmin biosynthesis gene (geoA gene) and 2-MIB biosynthesis gene by PCR, targeted gene were detected from 18 of 19 water sources. PCR targeting biosynthesis gene related to musty odor is a useful tool for survey of musty odor producing cyanobacteria in water sources as a monitoring system.
