FISH DIVERSITY DETECTION AT THE INNER PART OF OSAKA BAY USING ENVIRONMENTAL DNA ANALYSIS

Abstract

 Fish diversity at at Amagasaki canal in the Inner Part of Osaka Bay were investigated by environmental DNA analysis (eDNA) to clarify whether this method is valuable in eutrophic conditions. A ORP and DO was restored in January in Higashi-bori sampling point and the detected fish species was larger number than the other time. The result suggests that Higashi-bori has a potential for fish habitat with a water quality recovery. The number of detected species and fish fauna suggests that eDNA is efficient in comparison among sampling points which has sharp contrast of environmental factors and monitoring seasonal difference. The eDNA is unable to detected a certain species, but allow to detect larger number of species than the conventional sampling methods. eDNA is most effective when used in conjunction with conventional sampling method for investigation of fish diversity.