Genetic variation of Pinus densiflora and P. thunbergii using molecular markers （Ⅱ）

- Population differentiation of P. densiflora in Kangwon province of Korea -

Abstract

The population differentiation in seven natural populations of Pinus densiflora was studied based on RAPD markers. Four trees were sampled from each population. Six megagametophytic DNAs per tree were used as template DNAs for genotyping of a seed tree. Two hundred and fifty bands resolved in RAPD with twenty random primers were used to measure the genetic variation of population. The level of genetic diversity was relatively high (A=1.92, P₉₅=92.7, H₀=0.56, H_e=0.40) and the degree of genetic differentiation among populations (F_ST=0.135) was higher compared with those of other pines. The rate gene flow was estimated high ( N_m=1.604). The mean values of genetic distance (0.083) was a very low differentiation and a close genetic relationship in P. densiflora of Korea. Especially, we could find statistically significant relationship between genetic and geographic distances (r= 0.8337, P<0.001). The results obtained suggests that RAPD markers are valuable for the estimation of genetic diversity and for the study of the divergence among population in P. densiflora.