1985 Volume 11 Issue 2 Pages 217-222
The decomposition of uric acid by uricase obtained from yeast was studied experimentally in pH 7.0 tris·borate buffer. The effects of allantoin and hydrogen peroxide on the decomposition and the decay of uricase activity were observed as well as the decomposition rates. Experiments in the decomposition of uric acid were carried out at temperatures of 15, 25 and 35°C, at uric acid initial concentrations of 1.0×10-3, 3.0×10-3, and 5.0×10-3 mg/cm3 and at uricase initial concentrations of 3.7×10-4-7.4×10-3 mg/cm3, where the decomposition of uric acid were in the range of about 34-92 %.
The results are summarized as follows :
1) Allantoin and hydrogen peroxide showed no inhibition effect on the decomposition of uric acid. 2) The decay of uricase activity was expressed in fractional or linear form. 3) Parameters in the decomposition rate equation containing the term of enzyme activity decay were estimated by using observed data, and the time variation of uric acid concentration was simulated with these parameters to show good agreement with observed results.
