Partition Coefficients of a-Amylase in the Aqueous Two-Phase System Containing Dextran and Polyethylene Glycol

Abstract

The liquid-liquid equilibria of the aqueous two-phase system containing dextran (M_n= 24, 200) and polyethylene glycol (M_n=3000) were measured at 20°C. The partition coefficients of α-amylase (molecular weight= 50, 000) in this aqueous two-phase system were also determined at 20°C.
Further, the liquid-liquid equilibria and the partition coefficients were correlated using the Flory-Huggins equation and the osmotic virial equation. It was found that the osmotic virial equation could give a better correlation.