1995 Volume 21 Issue 1 Pages 158-165
To develop the design procedure for preparative liquid chromatography of macrolide antibiotics, parameters for the adsorption isotherms and for the ralated rate processes were estimated experimentally and the measured and calculated chromatograms were compared.
Erythromycin and erythromycin ethyl succinate were selected as a representative antibiotic and a model impurity, respectively. Parameters in Langmuir type adsorption isotherms and rate parameters for silica gel-organic solvent systems were obtained from the values of the moments for the single component chromatograms. Parameters for the adsorption isotherms showed that the extent of separation between solutes varied greatly with the kind and composition of the mobile phase. The mass transfer coefficients in the stationary and mobile phases were compared with the previous correlations. Binary chromatograms were calculated numerically with the use of. Markham-Benton type isotherms for the binary solutes and the values of the said parameters. The calculated and measured chromatograms agreed fairly well over all operating conditions.
In conclusion, it was recommended for the design of preparative liquid chromatography to calculate chromatograms of macrolide antibiotics by use of parameters estimated by the method presented here.
