1956 Volume 30 Issue 7 Pages 720-729
In the previous report the authors studied the combination of PANS No.610 with simple proteins and nucleic acid and discussed the effe: t of the combination process on the direct antiviral action of the medicament. The experimental results did not support the assumption, that the direct antiviral action of PANS No.610 might be due to its linkage to the above-mentioned substances. In the present report the influence of complex proteins on the direct action of PANS No.610 was examined with the following results.
1) PANS No.610 produced a combination product with lipovitellin, one of those compounds belonging to the lipoprotein group.
2) Lipovitellin exerted an inhibitory effect on the virus inactivation with PANS No.610.
3) The lauroyl group of PANS No.610 proved to be the essential radical to the linkage.
4) PANS No.610 affected lipid-protein bridges in lipoprotein.
5) The combination was reversible but more stable than that with simple proteins.
6) The antiviral activity of PANS No.610 was not influenced by added synthetic lipoproteins, such as cephalin-salmine complex or cephalin-clupein complex, with which the medicament went into a combination.
7) PANS No.610 could be linked to liponucleoprotein, prepared from the mouse liver, without a reduction in its antiviral activity.
Based on the experimental results obtained, the mechanism of antiviral action of PANS No.610 was defined as follows.
The linkage of PANS No.610 to constituent lipoprotein of virus body represents one of the underlying processes in the antiviral action of this medicament, while unspecific combination with simple proteins or nucleic acids does not partake in the antiviral mechanism.
It still remains to be studied, whether or not the direct antiviral action of the preparation depends solely upon the above-mentioned process. Other high molecular compounds of virus body must be likewise taken into consideration, and the problem will further be discussed in the following reports.
