Demonstration of the Biologically Produced Amines in the Stools of Ekiri Patients

Significance of bacterial enzymes in ekiri. I

Abstract

Histamine, agmatine, cadaverine, putrescine andry-aminobutyric acid were detected from the stools of 6 ekiri patients by the ion exchange resin method andpaperchromatography.
The stool was poured into the column of ion exchange resin “amberlite IRC 50 and” the absorbed substances were eluted with 2NHCl.
The eluted substances were again poured into the column of ion exchangeresin. “amberlite XE 64”, and the absorbed substances were eluted fractionally with aceticacidacetate buffer solution at pH 5.2. The final substances were condensed anddeveloped with butanol acetate on a filter paper and sprayed with ninhydrin.
The Rf values and colour of the spots indicated the existence of the abovementioned amines.
It was assumed that the activities of the amino acid decarboxylases of thepathogenic microorganism in the intestinal canal of ekiri patients might have produced the amines.
The significance of the amines as an exogenic factor in ekiri was discussed.